Skip to content
Login Register

#cell lines

2 public questions tagged with this topic.

Animal Tissue Culture 3

MRC-5 cells are derived from:

Death phase also called decline phase is final segment of in vitro growth curve where net cell number decreases as death rate surpasses division rate. Triggered by exhaustion of essential nutrients glucose and L-glutamine, severe acidification due to lactic acid production, accumulation of toxic ammonium from glutaminolysis, oxidative stress from reactive oxygen species generated by mitochondrial dysfunction, and activation of intrinsic apoptosis pathway involving Bax translocation, cytochrome c release from mitochondria, apoptosome formation with Apaf-1, and executioner caspase-3 cleavage of

Ref: Freshney Ch.13 Death phase decline apoptosis; Elmore S Toxicol Pathol 2007 Apoptosis intrinsic pathway caspase activation.

View Details
Animal Tissue Culture 1

Transformed cell lines usually:

Transformation to continuous cell line involves acquisition of genetic alterations bypassing normal senescence checkpoints that restrict primary cells. Spontaneous immortalization occurs rarely at frequency ten to minus seventh in rodent cells due to p53 mutation combined with telomerase reactivation via hTERT promoter mutation -124 C>T creating ETS binding site, viral oncogene mediated transformation using SV40 large T antigen binding and inactivating p53 tumor suppressor and retinoblastoma Rb releasing E2F transcription factors, human papillomavirus E6 binding p53 promoting degradation via u

Ref: Alberts MBoC transformation immortalization SV40 p53 Ras telomerase; Freshney transformed lines serum mitogens PDGF MAPK AKT dependence.

View Details