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#cell dissociation

3 public questions tagged with this topic.

Animal Tissue Culture 2

EDTA enhances the activity of:

Ethylenediaminetetraacetic acid is polyaminocarboxylic acid with hexadentate chelation property exhibiting high affinity for divalent cations calcium and magnesium. Many cell adhesion molecules are calcium-dependent: classical cadherins require calcium to rigidify extracellular repeats for homophilic trans-interaction, and integrins need divalent ions for conformational activation and ligand binding to RGD motifs in extracellular matrix. By sequestering calcium, EDTA disrupts these homophilic bonds, loosens adherens junctions, desmosomes, and focal adhesions, enhancing penetration of trypsin t

Ref: Alberts MBoC 6th Ed Ch.19 Cadherin Ca2+ dependent adhesion; Freshney EDTA chelates Ca2+ enhances trypsin activity for cell detachment.

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Animal Tissue Culture 1

Trypsin is mainly used in animal cell culture for:

Adherent mammalian cells deposit organized extracellular matrix and engage tissue culture surface through integrin mediated focal adhesions linking actin cytoskeleton to substrate, providing survival signaling through focal adhesion kinase FAK autophosphorylation tyrosine 397 recruiting Src, paxillin, vinculin, plus cadherin mediated cell-cell contacts dependent on calcium bridging extracellular domains EC1-EC2 homophilic binding. Subculture requires breaking these adhesions without irreversible damage to surface receptors. Trypsin serine protease belonging to PA clan S1 family purified from b

Ref: Freshney trypsinization Arg Lys cleavage integrin cadherin detachment; Alberts MBoC serine protease catalytic triad EDTA synergy.

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Cell Communication

Which enzyme is NOT used to dissociate cells in culture? (June 2019)

Separase, is consistent with established principles of cell signaling, receptor pharmacology and cellular regulation. Experimental measurements of binding parameters, genetic loss-of-function studies and pharmacological interventions all converge on the same interpretation. Related options address neighboring concepts but do not satisfy the precise criterion stated in the question.

Ref: NCERT Biology Class 11–12 Alberts et al Molecular Biology of the Cell Lodish et al, Molecular Cell Biology Cooper & Hausman, The Cell Abbas et al., Cellular and Molecular Immunology (for immunology sections)

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