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#cell culture medium

2 public questions tagged with this topic.

Animal Tissue Culture 1

Which medium has higher bicarbonate concentration?

Historical development of basal media reflects progressive understanding that increased nutrient concentration supports higher cell densities producing more lactate requiring stronger buffering to prevent acidification limiting growth. Eagle's Minimal Essential Medium MEM developed 1955 contained thirteen essential amino acids identified by omission studies, eight vitamins B group, glucose one gram per liter, salts Earle's with bicarbonate one gram per liter thirteen millimolar, designed for low density contact-inhibited fibroblasts like human diploid fibroblasts. RPMI-1640 formulated 1967 for

Ref: Dulbecco & Freeman DMEM high bicarbonate vs low MEM RPMI; Gibco Media comparison NaHCO3 1g 2g 3.7g levels CO2 requirement.

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Animal Tissue Culture 1

RPMI-1640 medium is commonly used for culturing:

RPMI-1640 originally developed by George Moore, Robert Gerner, and Herbert Franklin at Roswell Park Memorial Institute Buffalo New York 1967 for establishment of human lymphoblastoid cell line number 1640 from peripheral blood without fibroblast feeder layer, tailored specifically to suspension hematopoietic cells with distinct requirements versus adherent fibroblasts. Distinguishing features include low calcium 0.42 mM versus 1.8 mM in DMEM reducing cadherin mediated homotypic aggregation causing clumping and death of suspension lymphocytes, high inorganic phosphate 5.6 mM versus 1.0 mM suppo

Ref: Moore et al JAMA 1967 199:519 RPMI-1640 lymphocyte medium low calcium; Gibco RPMI handbook suspension immunology assays.

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