Lack of photosynthetic ability due to altered carbon metabolism is an example of:
Biochemical variation reflects lesions in primary and secondary metabolic pathways rather than chromosome morphology. In heterotrophic tissue culture, selection pressure for photolithotrophy is relaxed, allowing chlorophyll deficient sectors, carotenoid-less callus, or mutants defective in Calvin cycle enzymes Rubisco, phosphoribulokinase, and glyceraldehyde-3-phosphate dehydrogenase to proliferate. Altered carbon metabolism impairs sucrose independence, starch synthesis, or nitrogen assimilation via nitrate reductase and glutamine synthetase deficiency. Such lines require exogenous vitamins,
Ref: Taiz & Zeiger Plant Physiology 6th ed Ch 7 photosynthesis; NCBI NBK215354 carbon metabolism; Campbell Biology 12th Ch 10 auxotrophs; Bhojwani Plant Tissue Culture biochemical variation.
