MAS selects plants based on:
Marker-assisted selection replaces unreliable phenotypic screening with direct interrogation of DNA sequence polymorphisms linked to trait-controlling loci. Instead of measuring disease severity in field that depends on weather and inoculum load, MAS uses polymerase chain reaction amplification of SSR, SNP, or SCAR markers situated within few centimorgans of target gene, detecting presence of donor allele regardless of environment, plant stage, or dominance interactions. Segregating individuals are genotyped at seedling stage, selection made before flowering, accelerating backcross programs an
Ref: Tanksley SD 1989 BioTechnology – RFLP; Collard & Mackill 2008 Philos Trans – MAS based on DNA markers
