Stanley Miller under Harold Urey's guidance in early 1950s tested Oparin-Haldane hypothesis experimentally. His apparatus circulated methane, ammonia, hydrogen and water vapor past electric sparks simulating lightning, collecting products via condensation. Chromatography revealed amino acids such as glycine and alanine, demonstrating abiotic synthesis. The 1953 publication became landmark evidence for chemical evolution. Dating this experiment anchors timeline of experimental abiogenesis research. Therefore 1953 marks historic demonstration bridging theoretical chemogeny to laboratory verifica
Ref:
Alberts et al., Molecular Biology of the Cell, 6th ed., Chapter 1: Origin of Life.