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#bicarbonate

4 public questions tagged with this topic.

Animal Tissue Culture 1

Which medium has higher bicarbonate concentration?

Historical development of basal media reflects progressive understanding that increased nutrient concentration supports higher cell densities producing more lactate requiring stronger buffering to prevent acidification limiting growth. Eagle's Minimal Essential Medium MEM developed 1955 contained thirteen essential amino acids identified by omission studies, eight vitamins B group, glucose one gram per liter, salts Earle's with bicarbonate one gram per liter thirteen millimolar, designed for low density contact-inhibited fibroblasts like human diploid fibroblasts. RPMI-1640 formulated 1967 for

Ref: Dulbecco & Freeman DMEM high bicarbonate vs low MEM RPMI; Gibco Media comparison NaHCO3 1g 2g 3.7g levels CO2 requirement.

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pH and Buffer

Which of the following buffers is most important in human blood?

Phosphate buffer is the scientifically accurate answer to this question. Within the study of pH and Buffer, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Phosphate buffer directly address what is being asked. Among the other options, Carbonate buffer, Acetate buffer, and Borate buffer do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this to

Ref: Lehninger Principles of Biochemistry, Nelson & Cox, 8th Ed., Ch. 2

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