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#BCG vaccine

2 public questions tagged with this topic.

BCG vaccine is administered via:

BCG vaccine is administered via intradermal route which is uniquely specified among routine childhood immunizations. Technique involves injection of small volume 0.05 ml for infants under 12 months and 0.1 ml for older children into superficial dermis of lower insertion of deltoid muscle left arm using 26G or 27G needle inserted bevel upward at approximately 10-15 degree angle pushing needle 2 mm subepidermal creating characteristic peau d orange wheal 6-8 mm bleb that disappears within 30 minutes indicating correct placement. Dermis abundant in Langerhans cells expressing langerin CD207 and d

Ref: WHO BCG vaccine position paper 2018 intradermal; Plotkin BCG route; CDC intradermal.

Which organism is used for BCG vaccine?

BCG vaccine uses live attenuated organism Mycobacterium bovis originally isolated from cow tuberculosis lesion by Nocard 1902. Mycobacterium bovis is member of Mycobacterium tuberculosis complex sharing 99.9 percent sequence identity with Mycobacterium tuberculosis, causing zoonotic tuberculosis in cattle transmitted via milk. Albert Calmette and Camille Guérin at Institut Pasteur Lille attempted to attenuate starting 1908 by serial subculture on glycerinated potato medium with bile to reduce growth clumping, 230 passages over 13 years ending 1921. Attenuation associated with deletion of genom

Ref: Calmette Guerin BCG M bovis origin; Kaufmann Nat Rev Immunol 2020 BCG; WHO BCG Position.