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#APC/C-Cdh1

2 public questions tagged with this topic.

Cell Cycle Regulation

The APC/C-Cdh1 complex is required for:

Specificity and timing of APC/C activity arise from sequential association with two adaptors, Cdc20 and Cdh1, both containing seven WD40 repeats forming beta-propeller that binds D-box and KEN motifs. During prometaphase and metaphase, APC/C-Cdc20 polyubiquitinates securin and cyclin B to trigger anaphase and mitotic exit. At telophase, cyclin B degradation reduces CDK1 activity, allowing Cdc14 phosphatase to dephosphorylate Cdh1, enabling its binding to APC/C core. APC/C-Cdh1 then operates from late mitosis through entire G1, continuously ubiquitinating mitotic cyclins A and B, mitotic kinase

Ref: Peters, Nature Reviews Mol Cell Biol 2006, APC/C Mechanisms. Alberts 7th ed., Chapter 17.

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Cell Cycle Regulation

The role of APC/C-Cdh1 in cell cycle regulation is to:

Mitotic exit and establishment of G1 phase require elimination of mitotic cyclins that maintain high CDK1 activity and inhibition of microtubule dynamics regulators. While APC/C activated by Cdc20 initiates anaphase by degrading securin and partially degrading cyclin B, a second wave driven by APC/C bound to Cdh1, also known as Hct1, completes program. APC/C-Cdh1 recognizes distinct destruction box and KEN box motifs in substrates including remaining Clb2 and Clb5 cyclins in yeast, cyclin A and cyclin B1 in mammals, polo-like kinase Plk1, Aurora kinases A and B, and Cdc20 itself, targeting the

Ref: Visintin et al., Genes Dev 1998, APC/C-Cdh1 in Mitotic Exit; Peters, Nature Rev Mol Cell Biol 2006, APC/C Regulation.

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