In which microscopy are Moiré patterns deconvolved using algorithms?
SIM generates super-resolution by illuminating sample with striped patterns that create moiré interference with fine specimen details. Raw moiré images captured at multiple phases and angles contain high-frequency information folded into observable range. Computational deconvolution and Fourier transformation separate overlapping frequencies and reconstruct doubled-resolution image with ~100 nm lateral detail. Unlike STORM or PALM which localize single molecules, SIM relies on algorithmic processing of interference patterns. This reconstruction requires precise knowledge of illumination geomet
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.