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Question

Mammalian cells in active growth phase were seeded at a density of 1x10^5 cells/ml. After 72 hours, 1x10^6 cells/ml were obtained. The population doubling time of the cells in hours is (up to two decimal places) _________

Explanation

Calculation based on standard cell principles yields 21.67 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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