Practice question
Question
A microbial strain is cultured in a 100 L stirred fermenter for secondary metabolite production. If the specific rate of oxygen uptake is 0.4 h^-1 and the oxygen solubility in the broth is 8 mg/L, then the volumetric mass transfer coefficient (KLa) (in s^-1) of oxygen required to achieve a maximum cell concentration of 12 g/L is (up to two decimal places) _________
Explanation
Calculation based on standard cell principles yields 0.17 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
Discussion
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