Practice question
Question
If 10 µg of pure carbonic anhydrase catalyzes the hydration of 0.30 g of CO₂in 1 min at 37°C at Vₘₐₓ, what is the turnover number (kcₐₜ) of carbonic anhydrase (in units of min⁻¹)? (Mr of carbonic anhydrase is 30,000)
Explanation
In if 10 µg of pure carbonic anhydrase, 2.0 x 10⁷ min⁻¹ to 2.1 x 10⁷ min⁻¹ represents established outcome. functional assays confirms option B (2.0 x 10⁷ min⁻¹ to 2.1 x 10⁷ m) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
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