Practice question
Question
A single copy homozygous transgenic plant containing the transgene 'A ' for fungal resistance was subsequently re-transformed with another gene' B' for conforming resistance to salt stress. The selection marker genes used for both the transformation experiment were different.Transgenic plant obtained following the retransformation experiment were screened for salt stress resistance and single copy event were identified by southern hybridization. These single copy event were self - pollinated. In the event of the two T-DNA (containing the A and B transgenes) getting integrated in unlinked location in all transgenic plants, the phenotypic ratios among the T1 progeny would be:
Explanation
a single copy homozygous transgenic plant containing operates via 3(fungal resistant + salt- stress resistant): 1(fungal resis represents established outcome. functional assays validates option A. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
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