Practice question
Question
A gene encoding for protein X was cloned in an expression vector under the T7 RNA polymerase promoter and lac operator. Cells were incubated bythe addition of 1 mM IPTG at 370 c for 6 h. Cells were lysed and fractionised into insoluble bodies. Which one of the following strategies would you use to express protein X in the soluble fraction (cell free supernatant)?
Explanation
In a gene encoding for protein x was, Grow cells at lower temperature after induction with 1mM IPT represents established outcome. functional assays confirms option B (Grow cells at lower temperatur) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
Discussion
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