In which growth phase do bacteria prepare for sporulation?
Sporulation is energy expensive differentiation reserved for conditions where vegetative growth cannot continue. Throughout exponential phase nutrients abundant, ribosome content high, cells divide via FtsZ divisome and elongasome MreB. As population density rises and carbon nitrogen phosphate become scarce, quorum peptides ComX CSF and nucleotide second messengers ppGpp c-di-GMP accumulate, sensed by KinA KinB KinC histidine kinases. Kinases activate phosphorelay transferring phosphate from Spo0F to Spo0B to Spo0A master regulator. Phosphorylated Spo0A binds 0A boxes activating over 120 genes
Ref: Prescott's Microbiology, 11th ed., Chapter 7: Bacterial sporulation - Stationary phase Spo0A phosphorelay.