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Practice question

Question

You have purified an enzyme using a series of chromatographic methods. It was observed that a 10 µg mL ⁻¹ of this purified enzyme converted 10 mM substrate per hour at 25°C and pH 7. Its specific activity is _______ IU µg ⁻¹ . (rounded off to three decimal places)

Explanation

Calculation based on standard biochemistry principles yields 0.017 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.

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