Practice question
Question
You are performing a PCR reaction in which you need to use 20 pmoles of each primer. If both the primers are 20 nucleotides long and the average molecular weight of each nucleotide is 300 Da, the amount of each primer required for 100 μl reaction is:
Explanation
Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates 120 ng because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.
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