Practice question
Question
You are characterizing a new enzyme isolated and purified in the laboratory. If the maximum velocity of the enzyme is 1800 µmoles L ⁻¹ min ⁻¹ and the total concentration of the enzyme in the reaction mixture is 1.5 µM, then the turnover number of the enzyme is _______ s ⁻¹ . (answer in integer)
Explanation
Calculation based on standard biochemistry principles yields 20 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
Discussion
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