Practice question

Question

To investigate the relationship between microtubules and centrioles in fixed HeLa cells using an epifluorescence microscope, a researcher plans to conduct immunostaining using antibodies against tubulin and centrin ( centriolar protein). After the incubation with the primary antibodies and wash, she/he plans to use secondary antibodies that bind to the primary antibodies. Below is a list of secondary antibodies carrying various fluorophores (dyes) available to the researcher. A. Alexa 568 B . FITC C . Alexa 488 D . Alexa 647 Select the correct combinations of the appropriate dyes that the researcher would typically utilize to observe co‐localization in an epifluorescence microscope?