Practice question
Question
Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______. (Given: average molecular weight of each base pair is 660 Da)
Explanation
molecular techniques analysis shows vector design and amplification enable manipulation. Consequently Option NAT emerges as the valid choice since it utilizes validated laboratory methods, aligning with established principles in biotechnology literature.
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