Practice question
Question
The enzyme a-amylase used in starch hydrolysis has an affinity constant ( Km) value of 0.005 M . To achieve one-fourth of the maximum rate of hydrolysis, the required starch concentration in mM (rounded off to two decimal places) is ----
Explanation
Calculation based on standard biochemistry principles yields 1.67 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
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