Practice question
Question
In an experiment designed to clone a PeR amplified fragment in a cloning vector digested with Xho l ( CrrCGAG ) and Smal (CCC / GGG), whic:h one of the following combinations o f restriction enzymes can be used in the PCR primer to generate compatible ends for cloning ? (' /' indicates the site of cleavage within the recognition sequence)
Explanation
in an experiment designed to clone a operates via Sail (G/TCGAC) and EcoRV (GAT/ATC) represents established outcome. functional assays validates option C. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.
Related blogs
Guides and insights connected to this topic
Mendelian Inheritance (Mendelism): Mendel's Experiments, Laws of Inheritance, Modes and Deviations
Review Mendel's pea plant experiments, the fundamental laws of inheritance, different inheritance modes, and common deviations from classic Mendelian patterns.
Magnetic Effects of Electric Current Notes - Field Lines, Solenoid, Motor, Generator and Domestic Circuits
Explore how electric current creates magnetic fields, covering field lines, solenoids, motors, generators and domestic circuits.
Photosynthesis in Higher Plants Biology Notes - Experiments, Light Reaction, Calvin Cycle, C3 and C4 Pathway and Factors Affecting Photosynthesis
Study photosynthesis in higher plants, including early experiments, light reactions, the Calvin cycle, C3 and C4 pathways, and key factors.
Discussion
Comments
Please log in to join the discussion.
Login to commentNo comments yet. Be the first to start the discussion.