Practice question

MCQ

Question

In an effort to produce gene knockout mice, a gene targeted homologous recombination was tried, with the exogenous. DNA containing neor gene (confer G-4l8 resistance) and tkHsv gene (confers sensitivity to the cytotoxic nucleotide analog ganciclovir). If the neor gene was inserted within the target gene in the exogenous DNA and considering that both homologous and non-homologous recombination (random integration) is taking place, which one of the following statements is NOT correct about the possible outcome of the experiment?

Options

Choose one · Correct answer highlighted

Cells with non-homologous insertion will be sensitive to ganciclovir. Non-recombinant cells will be sensitive towards G-418 and resistant to ganciclovir. Homologous recombination will ensure that cells will be resistant to both ganciclovir and G-418. Homologous recombinants will grow in G-418 containing media but will be sensitive towards ganciclovir. Correct

Explanation

in an effort to produce gene knockout operates via Homologous recombinants will grow in G-418 containing media represents established outcome. functional assays validates option D. Other options propose substrate or regulatory direction, inconsistent with published kinetics and mutant phenotypes.

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