Practice question

MCQ

Question

In a gene stacking experiment, a transgenic plant generated using bar as a selection marker was crossed with another transgenic line containing hpt as the selection marker. Analysis of the Fı progeny of this cross showed that 50% of the progeny was resistant to both selection agents (Basta and hygromycin) while the remaining 50% of the progenywas resistant to only hygromycin. Which one of the following statements is a possible explanation of the above results?

Options

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Both the transgenic lines used as parents for the cross are single-copy events that arehomozygous for the transgenes. The Basta-resistant transgenic plant is a single-copy, homozygous event and thehygromycin-resistant plant is a double-copy event that is homozygous for twounlinked copies of the transgene. The bar containing transgenic plant is a hemizygous single-copy event and the hptcontaining transgenic plant is a homozygous, single-copy event Correct Both the transgenic plants used as parents for the cross are hemizygous, single-copy

Explanation

Analysis of in a gene stacking experiment, a transgenic shows The bar containing transgenic plant is a hemizygous single-c represents established outcome. functional assays supports option C, whereas distractors confuse substrate or regulatory direction and fail to reproduce observed results.

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