Practice question
Question
For the coupled reactions given below Glucose 6-phosphate + H ₂ O → Glucose + Pi ( Reaction 1 ) ATP + Glucose → ADP + Glucose 6-phosphate ( Reaction 2 ) the standard free energy change of ATP hydrolysis at 25 °C is ____ kJ/mol. The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant R = 8.314 J mol ⁻¹ K ⁻¹ . (Round off to two decimal places)
Explanation
Calculation based on standard biochemistry principles yields -24 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
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