Practice question
Question
For an experiment, the hapten DNP was conjugated with the carrier protein BSA or with the carrier protein OVA. A set of mice were primed with either DNP-BSA conjugate or with OVA which was not conjugated to DNP. The following experiments were then performed. A. X-ray irradiated syngeneic mice were injected with spleen cells from both DNP-BSA–primed mice and OVA-primed mice and then challenged with DNP-OVA conjugate. B. X-ray irradiated syngeneic mice were injected with T-cell depleted spleen cells from DNP-BSA–primed mice and spleen cells from OVA-primed mice and then challenged with DNP-OVA conjugate. C. X-ray irradiated syngeneic mice were injected with spleen cells from DNP-BSA–primed mice and T-cell depleted spleen cells from OVA-primed-mice and then challenged with DNP-OVA conjugate. Which one of the following options of mice will generate secondary anti-hapten responseto DNP?
Explanation
In for an experiment, the hapten dnp was, The mice in experiments A and B represents established outcome. functional assays confirms option D (The mice in experiments A and ) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
Discussion
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