Practice question
Question
Following are statements related to spectroscopic investigation of proteins. A. Tryptophan fluorescence in a protein is not sensitive to its environment. B. Observation of a large number of N; - Ni+1 connectivity’s in the NOESY spectrum of a protein suggests the presence of helical conformation. C. Only proteins with masses less than 5000 daltons can be identified by MALDI mass spectrometry. D. Protein conformation can be investigated by ESI mass spectrometry. Which one of the following options consists of both correct statements?
Explanation
In following are statements related to spectroscopic investigation, B and D represents established outcome. functional assays confirms option B (B and D) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.
Discussion
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