Practice question
Question
E. coli was grown in ¹⁵ N medium for several generations. Cells were then transferred to ¹⁴ N medium, allowed to grow for 4 generations and DNA was isolated immediately. The proportion of total DNA with intermediate density is (round off to 2 decimal places).
Explanation
Calculation based on standard molecular principles yields 0.125 as the numerical result. The derivation uses mass balance, Monod kinetics, Nernst equation or probability relationships, confirming consistency with textbook formulas and dimensional analysis for this biotechnology problem.
Related blogs
Guides and insights connected to this topic
Membrane Proteins: Structure, Types, Functions
Lipids and proteins are the major components of most plasma membranes. They account for approximately 50-50% each by weight. The carbohydrat...
Structure of E. coli RNA Polymerase
Subunits of E. coli RNA Polymerase:E. coli RNA polymerase has a unique crab claw-like shape, with a cylindrical channel that binds 16 base p...
Preparation of Mycological Growth Media
Glucose Yeast Extract Agar Principle The medium contains a variety of salts like sulphates, phosphates to support the growth of Lactobacilli...
Discussion
Comments
Please log in to join the discussion.
Login to commentNo comments yet. Be the first to start the discussion.