Practice question
Question
Digestion of a 5Kb linear DNA fragment with EcoRI generates two fragments of 2 Kb and 3 Kb, while digestion of the same molecule with HindIII yields three fragments of 0.7 Kb, 3.5 Kb and 0.8 Kb. When the same DNA is digested with both the enzymes, it yields fragments of 0.7 Kb, 1.3 Kb, 2.2 Kb and 0.8 Kb. The right sequence of restriction sites in the DNA fragment is
Explanation
Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates One EcoRI site in between two HindIII sites because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.
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