Practice question

The functional core promoter of RNA polymerase II extends approximately from minus forty to plus forty relative to transcription start site plus one, sufficient to direct accurate basal transcription in vitro with purified general factors. This minimal region integrates potentially TATA box at minus thirty, BRE elements upstream and downstream, initiator Inr spanning plus one, downstream promoter element DPE at plus twenty-eight to thirty-two, and GC-rich SP1 binding sites. Combinatorial presence dictates affinity for TFIID and positioning of polymerase active site precisely over initiator nucleotide for initiation.