Practice question

Question

An indusntrial fetmentor containing 10,000 L of medium needs to be sterilized. The initial spore concenn·ation in the medium is 10^6 spores mL^-1. The desired probability of contamination after sterilization is 10-^3. The death rate of spores at 121^ oc is 4 min^-1. Assume that there is no cell death during heating and cooling phases. The holding time of the sterilization process is min (rounded off to the nearest integer).