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Question

A recombinant protein is produced in Escherichia coli by two stage continuous cultivation at steady state. Upon induction of the culture in stage II, the specific growth rate of the culture decreased considerably. For an input flow rate in stage I of 100mL/h, a steady rate of product formation can only be obtained

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Explanation

Mechanistically, quantitative reasoning involves ratio, percentage and series logic determine correct calculation. This validates when stage I is smaller than stage II because it meets mathematical and logical criteria, a pattern repeatedly demonstrated in aptitude and reasoning research.

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