Practice question

Question

A fed batch process is running at quasi-steady state with respect to substrate and biomass concentration. At 2 h, the culture volume is 500 L with a constant sterile inlet feed at 50 L h-1 of glucose. The culture kinetic parameters μ𝑚and 𝐾𝑆 are 0.2 h-1 and 0.1 g L-1, respectively. The substrate concentration in the reactor will be ________ g L-1 (rounded off to one decimal place)