Practice question

MCQ

Question

Q143 Given below are the experimental protocols to find out the exact location of repetitive DNA sequence in mitotic chromosome by FISH (fluorescence in situ hybridization). Which one of the protocols will give the correct result?

Options

Choose one · Correct answer highlighted

Mitotic chromosomes were fixed on glass slide → incubated with biotinylated telomeric DNA → denatured →incubated with fluorescently labeled avidin → localization observed under fluorescence microscope. Mitotic chromosomes were fixed on glass slide → denatured → incubated with FITC labeled unrelated non- repetitive DNA sequence 4 counterstained with propidium iodide → localizati observed under fluorescence microscope. Mitotic chromosomes were fixed on glass slide → denatured → incubated with biotinylated satellite DNA3 incubated with) fluorescently labelled avidin → localization → observed under fluorescence microscope. Correct mitotic chromosomes were fixed on glass slide → incubated with repetitive DNA sequence binding protein → denatured → FITC labelled antibody against the protein → localization observed under fluorescence microscope.

Explanation

In q143 given below are the experimental protocols, Mitotic chromosomes were fixed on glass slide → denatured → represents established outcome. functional assays confirms option C (Mitotic chromosomes were fixed) as correct, while alternatives misstate substrate or regulatory direction, contradicting textbook data.

Practice question

Q143 Given below are the experimental protocols to find out the exact location of repetitive DNA sequence in mitotic chromosome by FISH (fluorescence in situ hybridization). Which one of the protocols will give the correct result?

Options

Explanation

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