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#stem cell classification

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Stem Cell -ll

Spermatogonial stem cells are classified as:

Spermatogonial stem cells reside along basement membrane of seminiferous tubules within specialized niche maintained by Sertoli cells producing glial-derived neurotrophic factor GDNF and fibroblast growth factor FGF2. In steady-state spermatogenesis they undergo self-renewing divisions to maintain pool and simultaneously produce differentiating spermatogonia that ultimately yield haploid spermatozoa through meiosis. Although capable of long-term self-renewal like other adult stem cells, lineage tracing demonstrates in vivo they generate only sperm lineage, and during prolonged culture can regain pluripotency if reprogrammed. Under physiological conditions they are classified as unipotent committed to male gamete lineage.

Ref: De Rooij & Russell, Reproduction 2000; Kanatsu-Shinohara, Cell 2003: Spermatogonial stem cells unipotent.

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Stem Cell -lll

Hematopoietic stem cells (HSCs) are classified as:

Hematopoietic stem cells isolated from bone marrow as lineage negative Sca1 positive cKit positive population sustain all blood lineages including myeloid granulocytes, monocytes, erythroid cells and lymphoid B and T cells but not extraembryonic or non-hematopoietic tissues, defining multipotency within hematopoietic system. They self-renew long-term repopulating irradiated recipients and serial transplantation, generate committed progenitors common myeloid progenitor and common lymphoid progenitor via transcription factors GATA1, PU.1, Ikaros. Totipotent generates entire organism including placenta, pluripotent all embryonic lineages, unipotent single lineage like spermatogonial stem. Multipotent classification reflects restricted potential to multiple lineages within one germ layer derived system maintaining lifelong hematopoiesis.

Ref: Orkin and Zon, Hematopoiesis, Chapter 2: Multipotent Hematopoietic Stem Cells Classification.

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