Practice question

Hematopoietic stem cells isolated from bone marrow as lineage negative Sca1 positive cKit positive population sustain all blood lineages including myeloid granulocytes, monocytes, erythroid cells and lymphoid B and T cells but not extraembryonic or non-hematopoietic tissues, defining multipotency within hematopoietic system. They self-renew long-term repopulating irradiated recipients and serial transplantation, generate committed progenitors common myeloid progenitor and common lymphoid progenitor via transcription factors GATA1, PU.1, Ikaros. Totipotent generates entire organism including placenta, pluripotent all embryonic lineages, unipotent single lineage like spermatogonial stem. Multipotent classification reflects restricted potential to multiple lineages within one germ layer derived system maintaining lifelong hematopoiesis.