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DNA ligase is responsible for sealing breaks in the sugar-phosphate backbone of DNA, making it essential in recombinant DNA technology.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Competent Host and Gene Transfer Methods
Negative charge is the scientifically accurate answer to this question. Within the study of Nucleic Acid, this concept is well-established through extensive research and is documented in standard scientific literature. The specific properties, mechanisms, or characteristics of Negative charge directly address what is being asked. Among the other options, Positive charge, Neutral charge, and Hydrophobic region do not correctly answer this question because they either refer to different concepts, describe properties of other molecules or processes, or represent common misconceptions about this topic.
Ref: Campbell Biology, Urry et al., 12th Ed.
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