14 public questions tagged with this topic.
DNA probes are used in Southern blotting to detect specific DNA sequences through hybridization.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Biosafety and Overview of Applications
Southern blotting requires gel electrophoresis to separate DNA fragments before hybridization.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting is a technique used to detect specific DNA sequences in a sample.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting relies on DNA hybridization to detect specific sequences.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting requires gel electrophoresis to separate DNA fragments before hybridization.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting is a molecular biology technique used to detect specific DNA sequences in a sample.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Processes - Isolation of DNA and Gel Electrophoresis
Southern blotting is classical technique established for specific DNA detection within heterogeneous genomic DNA population. Purified DNA is cut with restriction endonucleases, size-fractionated on agarose, denatured to single-stranded form, transferred to membrane via capillary action, and hybridized with labeled probe complementary to gene of interest. Visualization confirms presence, copy number, size, and rearrangement status of locus. Applications include mutation detection, RFLP mapping, and transgene integration analysis. In contrast, Northern blotting detects mRNA, Western detects proteins via antibodies, and lectin blotting detects carbohydrate moieties, demarcating Southern's DNA-specific role.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
Southern blotting developed by E. M. Southern transfers electrophoretically separated DNA fragments from agarose gel onto nitrocellulose or nylon membrane, preserving spatial arrangement of bands. Detection uses labeled single-stranded DNA or RNA probe that hybridizes to complementary sequences through Watson-Crick base pairing, specifically A-T and G-C hydrogen bonding under controlled stringency and salt concentration. Base pairing confers high specificity enabling identification of particular genes, RFLP alleles, and transgene integrations. Other principles like fluorescence may label probe, but underlying capture relies fundamentally on nucleic acid complementarity and hybridization kinetics.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
