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#pedigree breeding

2 public questions tagged with this topic.

F1 plants in pedigree breeding are not selected because they are:

F1 generation derived from a cross of two genetically distinct pure lines carries both parental genomes in heterozygous state, but every F1 individual receives exactly the same set of alleles: one allele from each parent at every locus. There is no segregation because all F1 plants originate from gametes of homozygous parents, so genotype Aa Bb is identical across the population. Consequently phenotypic variation within F1 is purely environmental, not heritable. Selection among identical genotypes yields no genetic advance, as predicted by breeder's equation R = h²S where genetic variance is zero. Additionally, F1 exhibits maximum heterozygosity and often heterosis, masking recessive traits that breeders aim to expose through segregation. Effective selection must wait until meiosis in F1 shuffles alleles, creating genetic variability in F2 through segregation and recombination, where distinct homozygous and heterozygous combinations become selectable and reveal transgressive segregation useful for further improvement and fixation. Breeder's equation shows zero selection response when genetic variance is absent, confirming that attempting selection in uniform F1 wastes resources and delays generation advance without creating new allelic diversity.

Ref: Hartl & Clark Principles of Population Genetics; Acquaah Chapter 4: F1 uniformity and heterozygosity

In pedigree breeding, selection starts from:

In pedigree breeding, segregation reaches maximum in F2 after crossing two homozygous parents to create heterozygous F1. F1 meiosis produces recombination through crossing over and independent assortment, generating new genotypic combinations in gametes. Fertilization of these gametes creates F2 individuals that vary widely for both oligogenic and polygenic traits, exposing recessive alleles and transgressive segregants exceeding parental ranges. Selection pressure cannot discern these recombinants in F1 because all F1 share identical heterozygous genotype. Starting individual plant selection in F2 therefore captures useful recombinants early, allowing fixation through subsequent selfing and progressive homozygosity. F2 population size is kept large, often 2000-3000 plants, to increase probability of recovering rare favorable gene combinations due to low frequency of desired allele linkages. Early selection preserves superior alleles before they are lost to random drift in bulk advancement and maximizes genetic gains. Recombination frequency and linkage disequilibrium decay influence recovery of novel alleles, and large F2 increases chance of breaking undesirable linkages to assemble favorable multi-locus haplotypes for improvement.

Ref: Simmonds NW & Smartt J. Principles of Crop Improvement, Chapter 6. NCERT Class XII Biology, Chapter 9