15 public questions tagged with this topic.
Gene cloning is a technique used to produce multiple identical copies of a gene for research, medicine,_x000D_ and agriculture.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Biosafety and Overview of Applications
Gene cloning is a process used to produce identical copies of a specific gene through recombinant DNA_x000D_ technology.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Biosafety and Overview of Applications
Cloning refers to the production of multiple identical copies of a gene or DNA segment.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Ethical Issues - GEAC and Animal Ethics
Gene cloning involves inserting a gene of interest into a host organism using vectors to produce multiple_x000D_ copies of the gene.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Transgenic Animals - Models and Biological Products
Transformation is the process of introducing recombinant DNA into a host cell for cloning.
Ref: NCERT Class 12 Biology Chapter 10: Biotechnology and its Applications Medical Applications - Molecular Diagnosis - PCR ELISA
Plasmids are circular, extra-chromosomal DNA molecules that replicate independently of the host genome.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Bioreactors and Downstream Processing
Ribosomes are involved in protein synthesis, not in DNA replication or vector construction.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Cloning Vectors - Plasmids Phages Cosmids Selectable Markers Ori
Cloning vectors must contain an origin of replication to allow independent replication in a host cell.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Cloning Vectors - Plasmids Phages Cosmids Selectable Markers Ori
A cloning vector must contain an origin of replication to allow it to replicate independently in a host cell.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Cloning Vectors - Plasmids Phages Cosmids Selectable Markers Ori
Selectable markers, such as antibiotic resistance genes, help in identifying and selecting recombinant cells.
Ref: NCERT Class 12 Biology Chapter 9: Biotechnology: Principles and Processes Principles of Biotechnology - Genetic Engineering
Recombinant DNA technology involves inserting foreign DNA into a host using a vector for genetic modification.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance Search for Genetic Material and RNA World
Determining insert orientation in a plasmid requires direction-sensitive detection. Amplification using one primer annealing within the insert and another annealing in the vector backbone produces a product only when the insert is in a defined orientation and yields size indicating forward or reverse cloning. Using two insert-specific primers merely confirms insert presence, independent of vector context. Single-site restriction digestion linearizes plasmid regardless of orientation, while two vector sites excise insert but do not distinguish its direction. This vector-insert PCR strategy is quick, reliable, and avoids sequencing for initial screening.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
