Which technique does NOT study conformational dynamics?
Techniques monitoring conformational dynamics must sense time-dependent structural changes: NMR chemical exchange and relaxation, circular dichroism thermal unfolding, fluorescence resonance energy transfer distance fluctuations, and single-molecule imaging. Mass spectrometry measures mass-to-charge ratio, providing stoichiometry, post-translational modifications, and sequencing via fragmentation, but conventional measurements occur in gas phase after ionization, losing real-time solution dynamics unless combined with hydrogen-deuterium exchange pulse labeling or ion mobility. Thus among options, mass spectrometry alone does not directly report continuous conformational dynamics in native aqueous environment relevant to enzyme catalysis and allostery.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.