Practice question

Gli family members Gli1, Gli2, Gli3 are zinc finger transcription factors homologous to Drosophila Cubitus interruptus mediating Hedgehog transcriptional response. In resting state, Suppressor of Fused Sufu sequesters Gli in cytoplasm, promoting sequential phosphorylation by PKA, GSK3, CK1 creating beta-TrCP degron generating repressor Gli3R that blocks targets like Patched1. Upon Hedgehog activation, Smoothened inhibits Sufu and PKA processing, allowing full-length Gli activators Gli1, Gli2A to enter nucleus, inducing Patched1, Gli1 itself, cyclins, Myc, Bcl2. TCF is Wnt effector, STAT cytokine effector, beta-catenin coactivator not Hedgehog specific, making Gli definitive readout.