Practice question
Question
An E. coli mating between Hfr trp+ his+ strS and F-trp- his- strR was allowed to proceed for 30 minutes. The mixture was plated on medium containing either (i) streptomycin + histidine or (ii) streptomycin + tryptophan. Replica plating revealed that in the first case ‘i’ 48 out of 104 colonies were his- while in the second case ‘ii’ 10 out of 70 colonies were trp-. Which of the following is the best representation of the location of the trp and his genes relative to the origin of transfer (>) of the Hfr chromosome?
Explanation
The principle of metabolic engineering and inheritance explains that exogenous gene introduction and pathway extension enable trait improvement. Hence ..........his........trp.....>....... fits best because it demonstrates metabolic extension and combinatorial biosynthesis, as documented in authoritative sources on genetics and biotechnology.
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