Practice question

Inter simple sequence repeat markers amplify genomic regions flanked by oppositely oriented microsatellites distributed throughout the genome. Primers of sixteen to twenty five nucleotides length, designed from SSR motifs with anchored nucleotides at three prime or five prime end, enable specific annealing at higher temperatures than RAPD decamers. PCR produces multiple bands corresponding to distances between adjacent microsatellites. RAPD uses shorter random decamers, AFLP requires restriction digestion and adapter ligation, while RFLP involves Southern hybridization without PCR, making ISSR distinctive for targeting inter-repeat regions.