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The Central Dogma states that DNA is transcribed into RNA, which is then translated into protein. Some exceptions, such as reverse transcription, exist.
Ref: NCERT Class 12 Biology Chapter 5: Molecular Basis of Inheritance Search for Genetic Material and RNA World
Appearance of larger RNA species exclusively after induction, visualized on Northern blot, strongly suggests regulated alternative splicing rather than degradation or genomic alteration. Controlled induction may activate specific splicing factors resulting in retention of intronic sequences or inclusion of additional cassette exons, increasing transcript length. Such larger products remain polyadenylated and intact, differing from degradative smears that show smaller heterogeneous fragments. Since probe targets same gene, genomic duplication would affect Southern blot pattern, not RNA size. Hence observation reflects dynamic modulation of splice site choice altering isoform composition.
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
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