Gene addition strategy in transgenic plants involves:
Transgenic improvement can proceed by supplementing genome with entirely new capabilities. Gene addition strategy introduces foreign coding sequences isolated from bacteria, other plants, or animals that confer traits absent in recipient, such as insecticidal Cry proteins, herbicide detoxifying PAT, or nutritional enzymes like phytoene synthase for provitamin A. Construct includes promoter, codon optimized coding region, transit peptide if chloroplast targeting required, and polyadenylation signal, delivered by Agrobacterium mediated T-DNA transfer or biolistics. Integration generally random,
Ref: Nicholl Genetic Engineering 4th ed Ch 6 addition; Watson Molecular Biology Gene Ch 12; NCBI NBK21530; ISAAA Pocket K 2 gene addition.
