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#transcription

87 public questions tagged with this topic.

CHEMISTRY

Which enzyme synthesizes RNA from a DNA template?

RNA polymerase catalyzes transcription, synthesizing RNA by reading the DNA template strand in the 3’ to 5’ direction. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Chemistry Textbook - Latest Edition for Academic Session 2026-27 (Rationalized Textbook for Class XI and XII)Topic: Mole concept, atomic structure, chemical formulas like H₂O, CO₂, CH₃CH₂NH₂ and periodic trends.

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BOTANY

Which of the following is NOT a function of RNA polymerase in eukaryotes?

RNA polymerase is responsible for transcribing RNA from DNA but does not synthesize DNA itself. This follows from latest NCERT 2026-27 principle explaining the concept clearly for NEET students in simple steps as per rationalized syllabus.

Ref: NCERT Biology Textbook - Latest Edition for Academic Session 2026-27 (Botany section, Rationalized Textbook for Class XI and XII), Chapter: Biology - Botany portion (Latest NCERT Textbooks for Academic Session 2026-27 -

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Microbial Physiology

Which antibiotic inhibits RNA polymerase, blocking transcription?

Bacterial transcription relies on a multisubunit DNA-dependent RNA polymerase holoenzyme core composed of two alpha, one beta encoded by rpoB, one beta-prime encoded by rpoC and one omega subunit plus sigma factor for promoter recognition. Rifampin belonging to rifamycin ansamycin class fits snugly into a deep hydrophobic pocket within the beta subunit located inside the main DNA-RNA channel but more than 12 angstroms away from the catalytic Mg2+ center that coordinates nucleotide addition. By occupying this pocket, the drug sterically obstructs the path for nascent RNA chains longer than two

Ref: Alberts et al., Molecular Biology of the Cell, 7th ed., Chapter 6: Bacterial RNA Polymerase and Rifampin Mechanism.

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