A test that measures soluble antibody with soluble antigen in gel is called:
When both antibody and antigen are soluble proteins, interaction can be visualized by allowing them to diffuse and form insoluble lattice in semi-solid medium. Immunoprecipitation encompasses methods where soluble immune complexes exceed solubility threshold and separate as precipitate, often facilitated by gel to stabilize band. Agglutination refers to particulate antigens like cells or latex beads, not soluble molecules. Radioimmunoassay employs radioactive labels for quantification, and flow cytometry measures cell surface fluorescence in suspension. Therefore detection of soluble-soluble r
Ref: NCERT Biology Class XII Principles on Klenow fill-in labeling, Lehninger Chapter 9 DNA cloning techniques, and Molecular Cloning by Sambrook Chapter 10 documenting end-labeling of cohesive termini.
