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#RNA interference

24 public questions tagged with this topic.

ZOOLOGY

Which technique uses RNA interference to silence specific gene expression in pests?

RNA interference (RNAi) is used to silence specific gene expression, including in pests for crop protection. This follows from NCERT principle where the relation explains the outcome clearly for students in simple steps.

Ref: NCERT Biology Textbook for Class XI and XII (Zoology section), Chapter: Principles of Inheritance, Molecular Basis of Inheritance and Biotechnology, Topic: Genetics, DNA techniques and applications.

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Transgenic Plant -2

Antisense RNA inhibits gene expression by:

Mechanism of antisense inhibition involves sequence-specific RNA-RNA interaction rather than protein-DNA binding. Transgene transcribes RNA identical to non-coding strand, complementary to mature messenger RNA of target gene. Inside nucleus or cytoplasm, antisense strand anneals to sense mRNA forming double-stranded duplex covering ribosome binding site or coding region. Duplex sterically blocks 40S ribosomal subunit scanning, preventing translation initiation. Additionally double-stranded RNA structure recruits RNase III like enzymes and RNA-induced silencing complex, leading to endonucleolyt

Ref: Green Annu Rev Biochem 1986 antisense duplex; Nellen Trends Biochem Sci 1993 mechanism; NCBI NBK21530 duplex blocking; Lodish Ch 8 antisense RNA action.

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Gene Therapy

Targeted inhibition of gene expression can be achieved by:

Antisense oligonucleotides synthetic single-stranded nucleic acids 16-20 nucleotides bearing phosphorothioate backbone replacing nonbridging oxygen sulfur increasing nuclease resistance albumin binding prolonging half-life, 2 prime O-methoxyethyl sugar increasing affinity Tm 2 degrees per modification reducing immune stimulation TLR7/8, locked nucleic acids constraining ribose C3 endo enhancing hybridization strength. Binding target mRNA via Watson-Crick pairing forming heteroduplex nucleus or cytoplasm, two mechanisms dominate: recruitment endogenous RNase H1 hydrolyzing RNA strand DNA-RNA hy

Ref: FDA Antisense Oligonucleotide Guidance 2023; Nature Reviews Drug Discovery Antisense Mechanism 2021; Alberts Molecular Biology RNA Targeting Chap 8.

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